KMID : 0613820080180020213
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Journal of Life Science 2008 Volume.18 No. 2 p.213 ~ p.219
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Evaluation of Proanthocyanidin Contents in Total Polyphenolic Compounds of Pine (Pinus densiflora) Needle Extracts and Their Antioxidative Activities
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Lee Ok-Hee
Kim Ki-Young Jang Min-Kyung Yoo Ki-Hwan Kim Sung-Gu Kim Mi-Hyang Lee Sang-Hyun
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Abstract
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Flavonoids in pine needles are known to be effective scavengers of free radical. Especially, proanthocyanidin, a kind of flavonoids possesses cardiovascular protection and antioxidative activities. Here, we evaluated proanthocyanidin contents in total polyphenolic compounds of pine needle extracts prepared by using hot water, ethanol, hexane or sub-supercritical . Analyses of total polyphenolic compounds and proanthocyanidin in each extracts indicated that hot water extract contained the highest concentrations, but sub-supercritical extract contained the lowest concentrations. On the other hand, evaluation of proanthocyanidin contents in total polyphenolic compounds in each extracts showed that sub-supercritical extract possessed the highest content, but hot water extract possessed the lowest content. These results indicate that extracts containing high concentrations of both total polyphenolic compounds and proanthocyanidin could be obtained by using hot water or ethanol extractions. Furthermore, extract containing high content of proanthocyanidin out of total polyphenolic compounds could be achieved by using sub-supercritical extraction. Measurement of antioxidative activities of extracts showed that hot water extract possessed the highest activity. In this study, we prepared extracts from pine needles by four different methods and evaluated the antioxidative compounds in extracts that could be used for effective components of functional food products.
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KEYWORD
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Antioxidative activity, pine needle, proanthocyanidin, total phenolic compounds
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